Changes in Soil Nutrient and Microbe Under Secondary Pinus massoniana Forests with Different Restoring Periods

doi:10.11924/j.issn.1000-6850.2014-0421

Abstract

Abstract: In order to study the relationships between soil nutrients and microbe in secondary Pinus massoniana forests with different restoring periods (10 a, 20-30 a and more than 50 a) in Guniujiang National Nature Reserve. The method of plate count and conventional chemical were used for determination. The results showed that: (1) Soil NH4 +-N had no significant difference (P＞0.05) in secondary Pinus massoniana forests with different restoring periods, while the other nutrients were mainly concentrated at 0- 20 cm and significantly different (P＜0.05), but the peak point distribution was different. (2) The order of the total number of soil microbes in different restore periods were: more than 50 a＞20- 30 a＞10 a, showed a significant difference (P＜0.05). The bacterium number was 20.11×10⁴- 521.11×10⁴ cfu/g, accounted for 50.25%- 89.43% , the highest proportion of the total soil microbe, followed by actinomycete and fungus. (3) Soil microbial biomass carbon (MBC), nitrogen (MBN), phosphorus (MBP) contents were significantly higher in Pinus massoniana forests with restore more than 50 a than that in the other forests (P＜0.05). (4) The contents of MBC and MBN in Pinus massoniana forests with different restoring periods were significantly and positively correlated with the total nitrogen (TN) and available phosphorus (AP). This indicated that soil TN and AP were the important factors affecting soil microbial biomass.

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