Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (20): 30-34.doi: 10.11924/j.issn.1000-6850.2014-0583

Special Issue: 水产渔业

• 23 • Previous Articles     Next Articles

Evaluate the Concentration of Purified White Spot Syndrome Virus with Spectrophotometry

qin chongtao   

  • Received:2014-03-07 Revised:2014-05-06 Online:2014-07-15 Published:2014-07-15

Abstract: First the quality of WSSV genome was calculated and the genomic DNA ratio of total WSSV particle mass was measured. According to the calculation, the mass of complete WSSV particles was 3.079×10-15g. In addition, the different components’proportion in WSSV dry weight was determined. The genomic DNA accounted for 10.17%, nucleocapsid accounted for 43.07%, envelop lipid accounted for 22%, envelop protein accounted for 24.76%. The relation between the concentration of WSSV and the OD600 of the suspension of WSSV is C=OD600×6.79×108virions/μL。