Abstract: Cercospora sojina, Phytophthora sojae and soybean root rot are the major pathogens of soybean, which can affect the quality and quantity of soybean products. To screen soybean cultivars and breeding lines with resistant to major pathogens is an effective way to control diseases. Resistances of 139 soybean cultivars to the pathogens of Cercospora sojina, Phytophthora sojae and soybean root rot were evaluated by artificial inoculation to screen resources with single or multi resistance. The results showed that 51 soybean cultivars and breeding lines possessed ability with high resistance to Cercospora sojina and Phytophthora sojae , 80 soybean cultivars and breeding lines with moderate resistance, 102 soybean cultivars and breeding lines with resistance to the pathogens Cercospora sojina and soybean root rot. 179 soybean cultivars and breeding lines were susceptible to three pathogens. 12 soybean cultivars and breeding lines shared traits with multiresistance to both Phytophthora sojae and soybean root, 14 soybean cultivars and breeding lines with mutiresistance to both Cercospora sojina and Phytophthora sojae, 16 soybean cultivars and breeding lines with multi-resistance to both Cercospora sojina and soybean root, 7 soybean cultivars and breeding lines with multiresistance to these three pathogens. The results indicated that, since a large part of soybean cultivars and breeding lines possessed single resistance to a pathogen and only minor soybean resources possessed ultiresistance to two more pathogens or three pathogens, rational utilization of soybean resources should be made.