Abstract: Pretreatment with diluted acid soaking and steam explosion were conducted for condition selection of bamboo saccharification effect. Using the content of revertose as measurement index, the author explored the concentration of diluted acid, soaking time, intensity pressure of steam explosion and the hold time of explosion pressure. The results showed that under the single factor experiment, the optimal condition and its corresponding revertose concentration were: when the soaking acid concentration was 2.5%, the concentration of revertose was 39.4301 g/L; when the soaking time was 10 h, the concentration of revertose was 35.2116 g/L; when the explosion pressure was 2 MPa, the concentration of revertose was 31.7554 g/L; when the hold time of explosion pressure was 150 s, the concentration of revertose was 30.3634 g/L. The optimal pretreatment condition obtained after orthogonal experiment: under room temperature, the bamboo was soaked in 2.5% vitriol for 8 h, and exploded after treated 180 s in 2 MPa steam explosion device. The highest output of revertose was 42.3707 g/L. And the primary and secondary order of the factors was the concentration of soaking acid> soaking time> the hold time of pressure> the pressure of explosion.