Abstract: The study aims to investigate the variations of physiological indexes of different foxtail millet varieties, and evaluate the strength of anti-senility of them. 5 foxtail millet varieties were used as the test materials to study he dynamic variations of the chlorophyll contents, the net photosynthetic rate (Pn), soluble protein contents, the SOD, POD, CAT activities and MDA contents of the leaves. The results showed that the changing trend of leaf physiological and biochemical indexes were consistent in the different foxtail millet varieties from flowering to maturing. The chlorophyll contents, the net photosynthetic rate (Pn) and the soluble protein contents of leaves gradually decreased 10 days after flowering, but the SOD, POD, CAT activities of leaves increased first and decreased afterwards, the contents of MDA increased 10 days after flowering. ‘An4585’＞‘Yugu17’＞‘Jigu14’＞‘Yugu11’＞‘Baogu18’was the anti-senility tolerance order of the foxtail millet varieties based on the leaf physiological indexes in 50 d after the varieties flowering by a comprehensive analysis of fuzzy membership functions. The research showed that the fuzzy membership function could evaluate the anti-senility of foxtail millet and our study could provide a reference for evaluating anti-senility of foxtail millet.