Molecular Characterization and Tissue Expression Analysis of PmTLR2 Gene from Pinctada martensii

doi:10.11924/j.issn.1000-6850.2014-0839

Abstract

Abstract: Toll like receptor 2 (TLR2) is an important component in the TLRs family, which is one family of pathogen-associated molecular pattern recognization receptors (PRRs). In this study, using rapid amplification of cDNA ends technology (RACE), we obtained the full length of PmTLR2 gene cDNA from Pinctada martensii (PmTLR2). The full length of PmTLR2 cDNA was 2614 bp, containing a 5’untranslated region (UTR) of 41 bp and a 3’UTR of 299 bp with 27 bp polyA tail. The open reading frame (ORF) was 2274 bp encoding 758 amino acid residues. Multiple sequence alignment indicated PmTLR2 had 51% sequence identity with that from Crassostrea gigas. SMART program analysis indicated PmTLR2 contained eight Leucine rich repeats (LRRs), one Leucine-rich repeat C-terminal (LRR-CT), one Leucine-rich repeat N-terminal (LRR-NT) and one TIR (Toll/IL-1R). Real-time PCR analysis demonstrated that PmTLR2 was constitutively expressed in all studied tissues (aductor muscle, gonad, hepatopancreas, mantle, hemocytes and gill) in P.martensii with the most abundant mRNA in the gill. These studies provide the basis for the further study of PmTLR2 in the immune response in mollusk.

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