Abstract: Salt stress is one of the most important abiotic stresses that limit the growth of plants and reduce crop yield and quality. Wild soybeans are wild relatives of cultivated soybean and possess many excellent genes. To evaluate the salt tolerance of wild soybeans, using float seedling technology and taking NaCl lethal concentration as evaluation index, 244 Glycine soja germplasms from Fujian Province were evaluated for salt tolerance at seedling stage. The results showed that the lowest and highest of NaCl lethal concentration were 200 mmol/L and 300 mmol/L, respectively. Among these germplasms, 14 with salt tolerance were screened out with NaCl lethal concentration of 290 mmol/L, representing 6% of the total test materials, only 2 with 300 mmol/L lethal concentration, accounting for 1%; 15 germplasms with salt sensitivity had 200 mmol/L lethal concentration, accounting for 6% of the total test materials. 36 test materials had lethal concentration of 270 mmol/L, accounting for 15%, and 32 test materials had lethal concentration of 220 mmol/L and 29 had lethal concentration of 280 mmol/L. In summary, wild soybean were significantly different in salt tolerance and rich in genetic variation, and could be expected to screen out excellent salt-tolerant germplasm for the study of salt tolerant QTL and mechanism.