Isolation and Diversity Analysis of Endophytic Fungi from Podocarpus macrophyllus and Davidia involucrata

doi:10.11924/j.issn.1000-6850.2014-0973

Abstract

Abstract: To investigate the diversity of endophytic fungi, 96 endophytes from P. macrophyllus and 87 strains from D. involucrata were isolated, respectively. All of 183 fungi were identified based on morphological characteristics and were further classified by restriction fragment length polymorphism (RFLP) method. 96 endophytes of P. macrophyllus were classified as Aspergillus, Colletotrichum, Phomopsis, Aphanocladium, Neurospora and mycelia sterilia. 87 endophytic fungi of D. involucrata were identified to be Aspergillus, Colletotrichum, Phomopsis, Penicillium, Xylariales sp. and mycelia sterilia. Aspergillus and Colletotrichum were the dominant genera in the endophytes from P. macrophyllus and D. involucrata. The community composition of endophytic fungi from P. macrophyllus was much similar to that from D. involucrata based on the similarity indices of Sorensen’s (CS) and Jaccard’s (CJ). Based on the RFLP typed results, endophytes from P. macrophyllus and D. involucrata were grouped into 22 branches. The similarity of strains in the same genus was higher than 93% while it was less than 83% between different genera, which was in accordance with the results of morphological classification. Some mycelia sterilia shared the same branch with the classified fungi but the others were much different from the known one in the dendrogram of RFLP pattern, which might be new genera. It suggested that the endophytic fungi isolated from P. macrophyllus and D. involucrate had plenty of diversity and could be a resource for further exploration in diversity of fungi.

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