Abstract: In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of 2 kinds of self-made Chinese herbal compounds (A&B) and 2 kinds of chemical bactericides (peroxyacetic acid andhydrogen peroxide, C&D) with 3 disinfection concentrations were compared in this study. The fertilized eggs of golden trout were randomly divided into 13 groups, each group with 2 parallels of 1000 eggs. Drugs at high, medium and low concentration were respectively used to immerse the fertilized eggs 35 min for disinfection once a day. The numbers of live and dead eggs were recorded each day before disinfection and the numbers of survival eyed eggs were counted in each experimental group after finishing the whole test. The results indicated that all of 4 kinds of drugs used here had a certain effect on prevention and control of saprolegniasis during hatching of fertilized eggs of golden trout, and also showed a correlation between the concentrations and effects. After the selection of several drugs, we found that group Bmedium, C-medium and D-high exhibited the most remarkable effects on control of saprolegniasis. However, careful consideration should be given to chemical drugs residues and their potential hazard to the eyed eggs. Therefore, Chinese herbal compound B was suggested to be used for prevention and control of saprolegniasis to achieve the healthy aquaculture. And the concentration and operation time of Chinese herbal compound B should be strictly controlled when used in disinfection of fertilized eggs.