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Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (28): 306-309.doi: 10.11924/j.issn.1000-6850.2014-1075

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The Detection of Mulberry Mosaic Dwarf Viroid and Analysis of Its Sequence Polymorphism

  

  • Received:2014-04-15 Revised:2014-04-15 Accepted:2014-08-06 Online:2014-10-15 Published:2014-10-15

Abstract: To detect the polymorphism of mulberry mosaic dwarf viroid, the sequence of mulberry mosaic dwarf viroid isolates in Ankang were investigated by RT-PCR, cloning and sequence analysis. The results showed that 4 variants of MMDVd were obtained, and the length was from 356 nt to 357 nt. The GC content was about 51% for all. There were about 58% pairs in MMDVd secondary structure predicted in Mfold web, and the two end of it were both branched.