Abstract: The HPLC detection method to detect the main effective components of the fermented liquid from Streptomyces corchorusii strain NF0919 was analyzed. By mycelium growth rate, the antibiotic and stability of different treatments fermentation liquid from strain NF0919 were studied. The results revealed that the most material composition was detected at the conditions of C18 column, 305 nm, gradient elution by methanol and water. The strain fermentation extract broths had instability to heat, alkali and uv, but was stable to acid, illumination. In the refrigerator storage condition, the antimicrobial activity of fermentation extract broths was stable, and the actinomycete strain NF0919 had certain research and development value.