Species Identification by mtDNA D-loop among Four Cyprinid Fishes

doi:10.11924/j.issn.1000-6850.2014-1128

Abstract

Abstract: In order to identify genetic variation and species identification between the cyprinid fishes, mtDNA D-loop was selected as a marker to determine mtDNA D-loop nucleotide sequence of four common cyprinid fishes (Ctenopharyngodoni dellus; Hypophthalmichthys molitri; Cyprinus carpio; Carassius cuvieri) in the study. The nucleotide composition A+T (averagely 71.4%) were much more abundant than G+C (averagely 28.6%) in the four kinds of fish. 132 polymorphic sites (22 singleton variable sites and 110 parsimony informative sites) was detected in 108 fishes . Based on the research, the authors defined 57 haplotypes (H1- H57) in these fishes. Haplotype diversity and nucleotide diversity were the highest in Carassius cuvieri (haplotype diversity: 1.000±0.017, nucleotide diversity: 0.03593) and the lowest in Ctenopharyngodoni dellus (haplotype diversity: 0.499±0.071, nucleotide diversity: 0.00817). The four kinds of fish could also be identified clearly by 53 characteristic nucleotides. According to the genetic variation analysis we could conclude that the haplotypes were different among four fish populations. No common haplotype was shared between them. Characteristic nucleotides could be a method used to identify four kinds of fish. Marking mtDNA D-loop can provide the germplasm resources conservation of fish and the authenticity of fish products.

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