Studies on Preparation of Extract from Ginkgo biloba Exotesta

doi:10.11924/j.issn.1000-6850.2014-1178

Abstract

Abstract: The resources of Ginkgo biloba exotesta, in which the phenolic acids have obvious inhibitory effect on crop diseases and insect pests, are rich in our country. In order to facilitate the development and utilization of these resources, in this study, we made a one- factor experimental design of solvent type, solvent concentration, ratio of material to liquid, extraction time and extraction temperature etc. respectively, for optimization of preparation system of extract of Ginkgo biloba exotesta (EGBE). Before this, the exotesta was collected when Ginkgo seeds were harvested, then was cleaned and naturally dried. The results indicated that EGBE with yield of up to 44.87% , in which ginkgolic acids were up to 13.25% , could be extracted and prepared by the optimized system including 80% ethanol, solid- liquid ratio of 1:20 (g:mL), ultrasonic extraction under the condition of 40℃ for 30 min. The above results not only provide EGBE with higher content of ginkgolic acids for prevention and control of plant diseases and insect pests, but also provide optimized system and techniques of EGBE preparation for efficient utilization of Ginkgo biloba exotesta.

References

[1] Teris A van Beek, Paola Montoro. Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals [J]. Journal of Chromatography A,2009,1216(11):2002-2032.
[2] 朱灿灿.银杏叶次生代谢产物的环境诱导机制及其调控[D].南京:南京林业大学,2010:1-2.
[3] 孙铭遥.基于紫外诱导银杏叶次生代谢产物及其差异蛋白质组学研究[D].杭州:浙江大学,2010:6-7.
[4] 陈鹏,韦军,余碧钰.银杏外种皮的利用价值[J].植物杂志,1996(3): 11.
[5] Chen Peng, Wang Li, Li Xiaoping, et al. Comprehensive Development and Utilization of the Exotesta Resourcesin Ginkgo biloba[A].见:陈鹏,林协.全国第十五次银杏学术研讨会论文集[C].北京:中国农业出版社,2007:34-42.
[6] 唐于平,楼凤昌,王欢.等.银杏外种皮的化学成分和药理作用[J].药学进展,2000,24(3):152-155.
[7] 韩帅,苗志伟,刘玉平,等.银杏外种皮挥发性成分分析[J].食品科学, 2012,33(14):146-149.
[8] 李稳宏,王军,李多伟,等.银杏外种皮制备生物农药提取工艺及应用研究[J].化学工程,2004,32(2):69-72.
[9] Pinelo M, Rubilar M, Jerez M, et al. Effect of solvent, temperature, and solvent- to- solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace[J].Journal of Agricultural and Food Chemistry,2005,53(6): 2111-2117.
[10] Banik R M, Pandey D K. Optimizing conditions for oleanolic acid extraction from Lantana camara roots using response surface methodology[J].Industrial Crops and Products,2007,27(3):241-248.
[11] Silva E M, Rogez H and Larondelle Y. Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology[J]. Separation and Purification Technology,2007,55 (3):381-387.
[12] 陆瑾,许忻,宗志敏,等.银杏制剂中烷基酚酸类成分研究进展[J].中草药,2001,32(1):85-87.
[13] 马国刚,王建中,卢晓蕊.响应面分析法优化槐米芦丁超声波提取工艺的研究[J].食品与发酵工业,2007,33(8):167-172.
[14] 杨小明,何军,柳艳君,等.银杏酸的荧光特征和光热稳定性研究[J].时珍国医国药,2009,20(10):2453-2454.
[15] 赵琛.银杏雄株叶片酚酸类化合物含量及其变化规律的分析研究[D] .扬州:扬州大学,2010:12-13.
[16] Wang Lili, Jia Yilv, Pan Zaifa, et al. Direct analysis of alkylphenols in Ginkgo biloba leaves by thermochemolysis–gaschromatography/ mass spectrometry in the presence of tetramethylammoniumhydroxide[J]. Journal of Analytical and Applied Pyrolysis,2009,85:66- 71.
[17] 薛治浦,李欣,朱文学.响应面法优化丹参叶总酚酸超声波辅助提取工艺[J].食品科学,2011,32(1):34-38.
[18] Wettasinghe, M&Shahid F. Evening primrose meal: A source of natural antioxidants and scavenger of hydrogen peroxide and oxygen- derived free radicals[J]. Journal of Agricultural and Food Chemistry,1999,47(5):1801-1812.
[19] 李小平.银杏外种皮有效成分的含量分析及其提取分离的研究[D].扬州:扬州大学,2005:55-57.
[20] 任星霖.银杏外种皮有效成分的代谢与 EGBE的制备研究[D].扬州:扬州大学,2006:47-50.
[21] Petr Ceslaa, Lars Blombergb, Mats Hamberg, et al. Characterization of anacardic acids by micellar electrokinetic chromatography and mass spectrometry[J]. Journal of Chromatography A,2006,1115(1- 2):253-259.