Abstract: In order to provide theoretical basis for the development and utilization of Ficus tikoua Bur on greening and farmland weed control, by using scanning electron microscope, the authors studied the microstructure of Ficus tikoua Bur, which was dealt with by glyphosate. The experiment results indicated that glyphosate could cause a periodical damage to the vegetative organ of Ficus tikoua Bur.; wither appeared at different degree of green leaf after certain time. Under the action of glyphosate, leaf epidermal cells were damaged; stomatal closure caused a serious effect on the leaf physicological function, such as photosynthesis and transpiration. The effect of glyphosate was more obvious to the tender leaves than the older ones. The research, which proves that glyphosate of bur, has certain killing effect, will guide the application of Ficus tikoua Bur. in landscape of urban areas.