Abstract: The available contents of microelements Fe, Mn, Cu, Zn and B were analyzed in 789 soil samples which were selected from different farm soil of Ganzhou District, Linze County, Gaotai County, Minle County and Sunan County in Zhangye area. The normal distribution test of Normal-QQplot was applied to eliminate 14 abnormal outliers. The 775 remaining data were analyzed using statistical ordinary Kriging interpolation test. In line with the spatial distribution of trace element and nutrient deficient critical value, five kinds of trace elements were divided according to the content spatial-distribution into three levels: rich, balanced and less. On the basis of different levels of fertility and crop nutrient deficiency symptom, the author concluded the influence of different fertility areas on agricultural products quality and put forward corresponding fertilization programs, in order to provide a reference for crop fertilizer application of Zhangye City.