Research on Effect on Biological Characteristics and Virulence of Beauveria bassianaInduced by Periostracum Cicadae

doi:10.11924/j.issn.1000-6850.2014-1289

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (34): 51-55.doi: 10.11924/j.issn.1000-6850.2014-1289

Research on Effect on Biological Characteristics and Virulence of Beauveria bassianaInduced by Periostracum Cicadae

Guan Bingbing¹, Wang Bing¹, Liu Yanwei¹, Meng Xinrui¹, Li Xiaohui¹, Zhang Jiashi²,
Xu Wenjing², Li Qiyun², Feng Shudan¹, Zhang Zhengkun²

(¹Harbin Normal University, Harbin 150025;
²Institute of Plant Protection, Jilin Academy of Agriculture Sciences, Gongzhuling Jilin 136100)

Received:2014-05-05 Revised:2014-09-17 Accepted:2014-10-14 Online:2015-03-10 Published:2015-03-10

Abstract

Abstract: Improved medium contained Periostracum Cicadae was used to induce the entomogenous fungus Beauveria bassiana, with the PDA medium as control. The growth rate, sporlation, spores germination and virulence against the third?instar?larva of Ostrinia nubilalis of the fungus were compared on both medium. The results indicated that sporlation, spores germination and bioactivity against pests induced by P. Cicadae were higher than that of the control, respectively, and showed significant difference. LT₅₀ of fungus induced by P. Cicadae against the pests was 4.73 days, and that of the control was 6.97 days. In addition, the sporulation time of the fungus induced and extracellular protease activity by improved medium was better than those of control group, The variation range of extracellular protease activity by improved medium was 1.497×10^-2-3.538×10^-2 IU/mL, but trowth rate of the former was lower than that of the later. In conclusion, the sporlation, spores germination, and virulence against pests were enhanced by P. Cicadae induction, it was potential for storage and improvement of B. bassiana strain.

关兵兵, 王冰, 刘艳微, 孟鑫睿, 李晓慧, 张佳诗, 徐文静, 李启云, 冯树丹 and 张正坤. Research on Effect on Biological Characteristics and Virulence of Beauveria bassianaInduced by Periostracum Cicadae[J]. Chinese Agricultural Science Bulletin, 2014, 30(34): 51-55.

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