Abstract: The soil pH value, nutrient level and microorganism amounts of tobacco field in Nanping, Fujian were analyzed. The results showed that the pH value of representative soil samples was less than 5.5, the concentration of organic matter, total nitrogen, alkali- hydrolyzale nitrogen were the highest in clay. The amounts of bacteria were higher than fungi and actinomyces. The correlation between bacteria and total phosphorous, available phosphorous, total potassium and available phosphorous were close; the fungi had negative correlation with total phosphorous, available phosphorous and total potassium, and had positive correlation with organic matter, total nitrogen, alkali- hydrolyzale nitrogen and available potassium; actinomyces had significant correlation with available phosphorous; phosphate- solubilizing bacteria had positive correlation with soil nutrient; potassium-solubilizing bacteria had significant correlation with available phosphorus and had extremely correlation with total phosphorous; the cellulose-utilizing bacteria was highly related to alkali-hydrolyzale nitrogen. The correlation between nutrient and microbes amounts in Nanping soil samples was not as strong as some other studies, which suggested that the weather condition, fertilization methods, especially the tobacco-rice crop rotation should be also important factors related to microbes growing. This study supplied more information about Nanping soil and also contributed to the better use of the microbiological fertilizer.