Abstract: The objective of this study is to investigate the nutrient absorption effects of compound fermentation liquid adding antagonistic bacteria 4-L-16 and T3-G-59 into 6 substrates on banana seedling. The effect fermentation liquid with antagonistic bacteria (4-L-16, T3-G-59) and without antagonistic bacteria (4-L-16, T3-G-59) on N, P, K, Ca, Mg accumulation of banana seedlings were analyzed under the potted condition. The results showed that all kinds of the fermentation liquids produced from different substrates played positive roles on the absorption of N, P₂O₅, K₂O, Ca, Mg. Further more, the fermentation liquid with the antagonistic bacteria added in them had much more significant effects on the absorption of P₂O₅, K₂O, Mg rather than the ones without, but had no significant effects on the absorption of N and Ca. From the experiment we concluded that bean cake, peanut cake or rapeseed cake could be the best fermentation substrates for 4-L-16 and T3-G-59.