Abstract: A soil-cultivating test, with rhizobag technique, was conducted to study soil enzymatic activities and its relationships with available heavy metal concentration in rhizosphere of rice (Oryza sativa L.). The changes of urea, phosphatase, sucrase and catalase activities in the rhizosphere soil and its relationships with available Cd, Pb, Cu and Zn concentrations were investigated. The soil samples were taken from the multimetal contaminated paddy soils due to the irrigation of acid mine drainage for more than 40 years. The results showed that both the pH value and urease activity in rhizosphere soil were lower than that in non-rhizosphere soil, while both the microbial biomass carbon concentration and phosphatase activity in rhizosphere soil were higher than that in non- rhizosphere soil. The concentration of available Cd and Zn in rhizosphere soil was lower than those of non- rhizosphere soil, and the concentrations of available Pb and Cu were higher in rhizosphere soil. Soil pH was significantly negatively correlated with the concentrations of available Pb and Zn; the concentrations of available Cd, Pb and Cu was significantly positively correlated with the concentrations of microbial biomass carbon; the concentrations of available Cd, Pb, Cu and Zn was significantly negatively correlated with soil urease activity; the concentrations of available Cd, Pb and Cu was significantly positively correlated with soil sucrase activity. Our results indicated that enzyme activities of soil were remarkably affected by available heavy metal concentration in multi-metal contaminated soil. The results provide theory evidence for controlling the heavy metal pollution of rice.