Abstract: The objective of this study is to investigate the effect of intercropping zingiberaceae crops with mature rubber on soil nutrients and soil enzymes. Soil nutrients and soil enzymes of mature rubber plantation with different intercropping Zingiberaceae crops were determined by conventional determination method. Results showed that effects of intercropping different Zingiberaceae crops in mature rubber plantation on total potassium, NO3-N (except intercropping ginger), pH value (except intercropping ginger), soil acid phosphatase activities, soil catalase oxidase activities (except intercropping Alpinia katsumadai) were little (P＞0.05). The organic matter content, total phosphorus, available phosphorus and total nitrogen (except intercropping Alpinia katsumadai) content and soil urease activities in mature rubber plantation soils significantly decreased after intercropping 3 species of Zingiberaceae crops. However, the NH4⁺-N content (except intercropping Amomum villosum), available potassium content and soil sucrase activities increased significantly. These results demonstrated that intercropping Zingiberaceae crops could improve the soil nitrogen and potassium supply capacity and decrease soil phosphorus supply capacity in short time. At the same time, these findings showed that in order to achieve the long-term success of intercropping in mature plantation, people must strengthen the crop fertilization to make soil nutrient balance, maintain soil function and protect soil quality.