Determination of phenolic compounds and assessment of antioxidant activity of oat bran by microwave-assisted extraction

doi:10.11924/j.issn.1000-6850.2014-1827

Abstract

Abstract: In order to set up a efficient method for extracting total phenolic compounds from oat bran, and reducing enviormental pollution, the Microwave-assisted extraction (MAE) was applied in the extraction and compared with traditional solvent extraction (TSE) as well. Effects of various conditions including the solvent, solvent to material ratio, microwave power and irradiation time on extraction yield of phenolics were investigated, the conditions was optimized by orthogonal design as well. The results showed that the optimal parameters for MAE were 75% of ethanol, 640 W of microwave power, 1:8 g/mL of solid-liquid ratio and 15 min irradiation time; under such conditions, 9.72 mg/g phenolic extraction yield was achieved with MAE, which was higher than 6.67 mg/g with TSE. The vitro antioxidant activity of the extract was evaluated by using DPPH assay. The extract with MAE had strong capacity of scavenging DPPH free radical, which was 90.4% as large as that of α-tocopherol.

武娇 and 任祎. Determination of phenolic compounds and assessment of antioxidant activity of oat bran by microwave-assisted extraction[J]. Chinese Agricultural Science Bulletin, 2015, 31(7): 229-234.

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