Gene Cloning and Bioinformatics Analysis of Two-Component Signal SystemsczcR2-czcS2 from Cupriavidus 6-5

doi:10.11924/j.issn.1000-6850.2014-1865

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (36): 148-154.doi: 10.11924/j.issn.1000-6850.2014-1865

Gene Cloning and Bioinformatics Analysis of Two-Component Signal Systems
czcR2-czcS2 from Cupriavidus 6-5

Shan Shiping, Guo Zhaohui, Zhang Deyuan, Liu Qingshu, Cheng Wei, Huang Jun, Wei Xiaowu,Wang Yushuang, Wu Shandong, Fu Zujiao

(Hunan Academy of Microbiology, Changsha 410009)

Received:2014-07-05 Revised:2015-01-09 Accepted:2014-08-08 Online:2015-03-20 Published:2015-03-20

Abstract

Abstract: In order to elucidate the Cd-resistance mechanism of Cupriavidus 6-5 and reduce the heavy metal pollution more efficiently. The two-component signal transduction system czcR2-czcS2 from high Cd- resistance strain Cupriavidus 6-5 was successfully cloned by homology-based cloning method. Results of DNA sequencing and sequence alignment showed that the identity of the nucleotide sequence of obtained genes czcR2 and czcS2 between Cupriavidus 6-5 and the megaplasmid of Cupriavidus metallidurans CH34 were from 99% and 98%, respectively. czcR2 and czcS2, adjacent to each other and the same direction of transcription, control other functional gene expression probably as an independent operon. The general characteristics, signal peptide and transmembrane domain of the regulatory gene were predicted by the bioinformatics. The study establish foundation for the development of more thorough research and making of heavy metal pollution control countermeasure, the work has the theory significance and actual application potential.

Shan Shiping,Guo Zhaohui,Zhang Deyuan,Liu Qingshu,Cheng Wei,Huang Jun,Wei Xiaowu,Wang Yushuang,Wu Shandong and Fu Zujiao. Gene Cloning and Bioinformatics Analysis of Two-Component Signal Systems
czcR2-czcS2 from Cupriavidus 6-5[J]. Chinese Agricultural Science Bulletin, 2014, 30(36): 148-154.

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Gene Cloning and Bioinformatics Analysis of Two-Component Signal Systems
czcR2-czcS2 from Cupriavidus 6-5

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