Advances on the Isolation of Protoplast in Gramineous Plants

doi:10.11924/j.issn.1000-6850.2014-1921

Abstract

Abstract: The protoplast is a convenient tool for genetic improvements and characteristic researches on cell physiology and biochemistry in gramineous plants. In order to provide theoretical basis for establishment of protoplast isolation in gramineous plants and promote the application, a detailed description about protoplast isolation research progress in gramineous plants from four aspects, including materials, pretreatment, the methods and conditions of separation as well as purification, were reviewed in the article. The relevant factors affecting the protoplast separation were analyzed. At the same time, some suggestions about the protoplast separation were put forward and utilization of gramineous plants was also proposed.

Peng Xiaoqun,Tang Ran and Xie Xinming. Advances on the Isolation of Protoplast in Gramineous Plants[J]. Chinese Agricultural Science Bulletin, 2015, 31(1): 252-257.

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