Abstract: The study aims to define the types and features of the key enzymes secreted by herbaceous fiber biological extraction strains. The activities of pectinase, mannanase and xylanase secreted by herbaceous fiber bio- extraction strains were systematically determined using the classic DNS method. The results showed that pectinase, mannanase and xylanase were simultaneously secreted by the herbaceous fiber bioextraction strains. During the fermentation in 9- 11 h, the pectinase activity of strain CBXW- 3 was the highest in the six target strains, up to 123 IU/mL, followed by that of strain CBXW- 1, 105 IU/mL. The mannanase activity of strain CBXW-4 was the highest, up to 214.2 IU/mL, and the next was that of strain CBXW-1, 162.1 IU/mL. Moreover, the xylanase activity of strain CBXW-4 was the highest in the six object strains, up to 111.4 IU/mL, and followed by that of strain CBXW- 6, 87.6 IU/mL. The varieties and the activities of key enzymes secreted by the six herbaceous fiber bio-extraction strains were better than that of the similar reports. It may provide scientific basis for further clarification of herbaceous fiber bio-extraction mechanism and study on the application value of the microbiology resources.