Abstract: To provide reference for selecting suitable late rice varieties for acidified paddy field, a germination experiment was conducted under 6 pH levels to compare the acid-tolerance of 25 late rice varieties at seed germination period by examining the germination rate, seed germination potential and fresh weight per seedling. The results showed that: (1) there were clear difference in germination rate, seed germination potential and fresh weight per seedling among varieties, in which those of No.3, 7, 10 and 15 variety were higher, and those of No.18, 19, 21 and 22 variety were lower; (2) the change extent of germination rate, seed germination potential and fresh weight per seedling of No. 6, 11, 12, 13, 14, 16, 19, 21, 23, 24 and 25 under 6 pH levels were bigger, so these varieties belonged to acid-sensitive varieties; (3) the change extent of germination rate, seed germination potential and fresh weight per seedling of No. 2, 3, 5, 10, 15 and 17 under 6 pH levels were smaller, so these varieties belonged to acid non-sensitive varieties. Comparison on acid-tolerance of seeds at germination stage can provide important reference for preliminary screening of suitable rice varieties for acidified paddy field.