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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (3): 249-252.doi: 10.11924/j.issn.1000-6850.2014-2148

Special Issue: 生物技术

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ISSR Analysis on Sorghum-White Hull Sudangrass of Cross F1 and Its Chromosome Doubling Plants

  

  • Received:2014-08-06 Revised:2014-08-06 Accepted:2014-09-09 Online:2015-03-19 Published:2015-03-19

Abstract: The paper aims to clarify genetic differences and genetic distance of sorghum-white hull sudangrass of cross F1 and chromosome doubling plant of sorghum-white hull sudangrass hybrids F1 and lay the foundation and provide a scientific basis for further breeding of new varieties. ISSR markers for sorghum- white hull sudangrass of cross F1 and chromosome doubling plant of sorghum-white hull sudangrass of cross F1 were used to carry out the comparative analysis of polymorphic loci. The results showed that, ISSR markers were amplified with 6 suitable primers and 164 bands were obtained, among which the bands of polymorphic were 144, accounted for 86.9%. The genetic distance was ranged from 0.2690 to 0.5923, and the genetic distance between sorghum and doubling plant of sorghum-white hull sudangrass of cross F1 was further. Using the genetic distance value of 0.26 as threshold, the test materials could be divided into 3 kinds: sorghum and white hull sudangrass, sorghum- white hull sudangrass of cross F1 and doubling plant of sorghum- white hull sudangrass of cross F1.