Abstract: The study aims to evaluate objectively, scientifically, fair and impartially the high yield ability, genetic stability and adaptability of varieties of summer soybean in Henan Province regional trial. Regional Crop Trials Statistical Analysis System (RCTAS) was adopted to analyze and compare the high and stable yield of soybean varieties according to the summarized data from the regional trial in Henan Province in 2013, in order to provide a theoretical basis for the effective utilization of new soybean varieties in Henan in production and breeding. The results showed that nine new soybean varieties, including ‘Qiule 1205’, ‘Andou 5156’, ‘Zhudou 02-19’ and so on, obtained high yield which were 5% higher than that of the control ‘Yudou NO.22’; ‘Nongfeng18’, ‘Zhudou 03-56’ and ‘Zhoudou 22’ had high and stable yield while the control ‘Yudou NO.22’ had stable yield; the yield of ‘Fan 09C6’ was poor, but stability was good. The test results combined with actual performance of new soybean varieties in the field prove that RCTAS is the excellently designed statistical analysis software with close integration of theory and practice, and can be conveniently applied to regional crop trial. The experimental results also provide scientific basis for identification, screening, examination and popularization of new summer soybean varieties in Henan Province.