Abstract: The effect of three plant growth regulators on machine-transplanted early hybrid rice seedlings and yields was evaluated by comparing the growth characteristics of the early hybrid rice variety‘Ganxin203’. The results showed that, in comparison with the control, all three growth regulators caused dwarfing in rice seedlings, and increased the pseudo stem width, green leaf and root numbers, the soluble sugar and total nitrogen contents. Meanwhile, the regulators improved the ability of tillering and adult seedling rate, and guaranteed the quality and yield of machined- transplanted seedlings. Among the three regulators, paclobutrazol had the best effects, gained the highest adult seedling rate and the highest yield, which increased by 3.74% and 9.98% respectively compared with the control. Comprehensively, growth regulators were benefit for obtaining stronger seedlings and higher yield; paclobutrazol was an appropriate regulator for machinetransplanted early hybrid rice seedling.