Abstract: The study aims to investigate the implantation of exogenous SO₂ on Perilla frutescens seeds germination and seedling membrane physiology and biochemical. The research used various concentrations of SO₂ (0, 0.5, 1, 1.5, 2, 2.5 mmol/L) to treat Perilla frutescens seeds to germinate under appropriate conditions, and determine its seeds germination rate, germination potential and germination index, as well as chlorophyll, MDA (malondia idehyde), POD (peroxidase), PPO (polyphenoloxidase), soluble sugar content of seedlings. The results showed that with the SO₂ concentration increased, the germination rate, germination potential, germination index, POD activity and soluble sugar content were first increased and then decreased, the maximum reached 1.5 mmol/L. Chlorophyll content generally showed a gradual upward trend with the increase of SO₂ concentration. MDA content, PPO activity along with the increase of SO₂ concentration, a generally upward trend after the first fall, were lowest in 1.5 mmol/L. What the figure showed that in the SO₂ concentration of 1.5 mmol/L was most favorable for Perilla frutescens seeds germination and growth, and could improve seeds antioxidant stress capacity.