Abstract: In order to reveal the distribution of organic matter contents in tobacco growing soils and its influencing factors in Longhui, spatial distribution of organic matter contents, and the distribution influential factors, such as soil type, soil texture, pH and so on, were analyzed. Results showed as follows: (1) organic matter contents in the tobacco soil were higher for tobacco cultivation with amplitude of 5.37-86.46 g/kg and variation coefficient of 28.09%, organic matter contents of about 21.09% of the soil samples were fit for tobacco cultivation; (2) significant differences were found among the different villages and towns for the contents of organic matter, the soil organic matter contents in the 6 main tobacco planting villages and towns were higher, and of medium variability and low variability; (3) significant differences were found among the different soil types, the contents of organic matter from red soil and yellow soil had a suitable level, and other types of soil had a higher contents of organic matter; (4) significant differences were found among the different soil texture, the average organic matter content in the tobacco soil was 32.03-34.21 g/kg; (5) significant differences were found among the different soil pH, the organic matter contents increased with the rise of the pH value, and the organic matter contents was higher in soil which had pH＞7.0 in Longhui.