Abstract: Potato virus A (PVA) is a kind of quarantine pest which can infect potato and cause the losses of yield and value of potato. In order to prevent the pest from entry and spreading, the one step loop-mediated isothermal amplification (RT-LAMP) method for the detection of potato virus A (PVA) was developed. Using primer explorer software, RT-LAMP primers were designed originally using a conserved region in the coat protein coding region of PVA. The amplification curve result on specificity using PVA, PVV, PVY, LMoV, negative control and water as template showed that the assay could amplify PVA specifically, which was same the fluorescent detection reagent (FDR) reaction result. The sensitivity comparison showed that the RT-LAMP was 10 times more sensitive than conventional reverse transcriptase polymerase chain reaction (RT-PCR) using serial dilutions of total RNA extracts. The method can be used to detect PVA rapidly on site.