Study on Sperm Cryopreservation of Three Rare Migratory Fishes in the Yangtze River

doi:10.11924/j.issn.1000-6850.2014-2464

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (5): 55-58.doi: 10.11924/j.issn.1000-6850.2014-2464

Special Issue: 水产渔业

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Study on Sperm Cryopreservation of Three Rare Migratory Fishes in the Yangtze River

Wang Minghua¹, Zong Liqiang¹, Chen Youming¹, Ding Shuyan¹, Qin Qin¹,

Bian Wenji¹, Cai Yongxiang², Chen Xiaohui¹（¹Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017; ²Institute of Oceanology & marine Fisheries, Nantong jiangsu 226007)

Received:2014-09-11 Revised:2015-02-02 Accepted:2014-10-30 Online:2015-03-20 Published:2015-03-20

Abstract

Abstract: The technique of sperm cryopreservation of three rare migratory fishes in the Yangtze River was studied, including Acipenser baerii, Alosa sapidissima and Takifugu obscurus. The success of sperm cryopreservation was evaluated by post-thaw sperm motility. To explore methods for sperm cryopreservation, this study evaluated the effects of different extenders, cryoprotectants, their volume fractions, equilibration time and dilution ratios. The result showed that the best sperm cryopreservation of motility of Acipenser baerii was (45±5.00)% and Takifugu obscurus (60.5±2.69)% were obtained by following procedure: the ringer solution and the semen were mixed at ratio of 2:1 in volume and added 10% methanol, while the best sperm cryopreservation of motility of Alosa sapidissima (80.3±2.58)% was obtained by following procedure: the ringer solution and the semen were mixed at ratio of 3:1 in volume and added 10% methanol. Sperms were cryopreserved by three-step cooling progam. These results provided the methods to conserve genetic resources in three rare migratory fishes in the Yangtze River.

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Study on Sperm Cryopreservation of Three Rare Migratory Fishes in the Yangtze River

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