Abstract: Distribution characteristics of zooplankton biomass and its correlation with water environmental factors were investigated and analyzed in four ponds where dominant species were Oxyeleotris marmoratus, Ctenopharyngodon idellus, Micropterus salmoides and Channa argus respectively at the Pearl River Delta region from May to December in 2012. Results have shown that throughout the investigation period, there was no significant change in distribution of zooplankton biomass of the four ponds, and the average values of biomass were 4.718 mg/L, 3.693 mg/L, 2.579 mg/L and 20.932 mg/L, respectively. Biomass of microzooplankton (protozoa and rotifers) accounted for a very high proportion of the total zooplankton biomass in the four ponds, while cladocerans and copepods contributed relatively less to the total zooplankton biomass. Correlation analysis showed that, in the Oxyeleotris marmoratus dominant pond, the environmental factors such as pH value, water temperature, transparency and total solids content had a significant impact on distribution of zooplankton biomass. In the Ctenopharyngodon idellus dominant pond, water temperature, transparency and total solids content were the main environmental factors affecting the distribution of zooplankton biomass. In the Micropterus salmoides dominant pond, dissolved oxygen content greatly affected the distribution of zooplankton biomass. In the Channa argus dominant pond, pH value, water temperature and nitrite content significantly affected the distribution of zooplankton biomass. One-way analysis of variance (ANOVA) results showed that there was very significant difference between the average values of zooplankton biomass in the four ponds (p < 0.001).