Abstract: The influence factors of plant male sterility were system reviewed in this paper, including genetics, pollen development in various periods mutations were likely to cause male sterility; the metabolism related enzymes, especially the active oxygen scavenging enzymes, such as peroxidase, catalase and cytochrome oxidase, etc; the hormones include auxin, gibberellin, cytokinin, abscisic acid and ethylene, etc; the content of mineral elements, soluble sugar and protein; the external environment factors, such as temperature, illumination condition, etc. Influencing factors on plant male sterility were summarized for providing a theoretical basis for male sterility depth study.