Abstract: Exploration of paclobutrazol and chloride on growth of lily plantlets optimal concentration, improve quality and transplant survival rate of test-tube plantlets of Oriental Lily. This test using test-tube plantlets of Oriental Lily as test materials, setting single-factor variables, respectively in MS basal medium with different concentrations of PP₃₃₃ (0.5, 1.0, 2.0, 3.0mg/L) and CCC (5, 10, 15, 20mg/L), in the control group without any plant growth retardants. Explore the most appropriate Lily plantlets growth and concentration of PP₃₃₃ and CCC. The result indicates the appropriate PP₃₃₃ and CCC can increase the concentration of rooting and adventitious bud differentiation of lily plantlets number, plant height, blade increase, well developed roots. But after the high concentration, blade negative volumes, or even yellowing phenomenon. Best fit for Oriental lilies grown PP₃₃₃ concentration is 1.0mg/L, CCC is for 10mg/L.