Responses of Soil Nutrients and Microbial Biomass and Community Composition to Long-term Fertilization in Cultivated Land

doi:10.11924/j.issn.1000-6850.casb14100042

Abstract

Abstract: To study the influences of long-term different fertilization on soil fertility and microbial community, a long-term fertilization experiment based on wheat-rice rotation agricultural system was conducted in Hang-Jia-Hu plain. The experiment included five fertilization treatments and a control without application of any fertilizers. The five different fertilization treatments were inorganic fertilizer (NPK), straw (Straw), organic manure (Manure), inorganic fertilizer plus straw (S NPK), and inorganic fertilizer plus organic manure (M NPK). The phospholipid fatty acid technique was used to characterize the soil microbial biomass and community. Results indicated that all of fertilization treatments increased soil organic carbon, as compared with control. Contents of organic carbon in the soils applied with S NPK, manure, and M NPK were significantly higher than that of the control. Application of chemical fertilizer, manure, S NPK and M NPK increased total N, total P and available K in the soils in a certain extent. Application of straw or organic manure significantly increased soil total PLFA, as well as bacterial, fungal, actinomycete PLFA and Shannon indices (P＜0.05). Application of chemical fertilizer decreased soil microbial biomass PLFA. Pearson’s correlation analysis indicated that soil microbial biomass PLFA and Shannon indices were significantly correlated with soil C/N ratio (P＜0.05). The result of Canonical Correspondence Analysis indicated that soil organic carbon content (F=2.18, P=0.027) had significant contribution to the variation of soil microbial communities. It can be concluded that long-term of straw incorporation or organic manure application can benefit soil microbial biomass and change soil microbial community. Soil organic carbon and C/N ratio can have important impact on soil quality.

Wu Qifeng,Lu Kouping,Mao Xiali,Qin Hua and Wang Hailong. Responses of Soil Nutrients and Microbial Biomass and Community Composition to Long-term Fertilization in Cultivated Land[J]. Chinese Agricultural Science Bulletin, 2015, 31(5): 150-156.

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