Effects of Compound Chinese Herbal Medicine on Non-specific Immunity of Amur Sturgeon

doi:10.11924/j.issn.1000-6850.casb14100054

Abstract

Abstract: To screen appropriate immune enhancer of Chinese herbal medicine for Acipenser schrenckii, the effects of three kinds of compound Chinese herbal medicine on non-specific immune Amur sturgeon were studied. There were three test groups and one control group. Each group was subdivided into 2 replicates with 200 fish per replicate. Three kinds of compound Chinese herbal medicine were added with the amount of 2.5% of the body weight respectively in the basal diet to feed healthy, non-immune 1 age Amur sturgeon for 35 days. The control group was fed by diet without compound additives. When the feeding experiment finished, serum, liver, spleen and kidney tissues were collected for investigating the immunity indices of Amur sturgeon. The results showed that the contents of complement 3 and complement 4 in serum in the three experimental groups were higher than that in the control group, and the contents of complement 3 and complement 4 of compound 3 were significantly higher than that in the control group(P＜0.05). Content of lysozyme in serum of compound 3 also increased obviously(P＜0.05), but contents of lysozyme of compound 1 and compound 2 were not significantly higher (P＞0.05). Contents of lysozyme in compound 1 and compound 2 were also higher in liver and spleen but were not significant(P＞0.05). Contents of NO of compound 1 and compound 3 in serum were obviously higher than that in the control group(P＜0.05). The activity of NOS in spleen did not increase significantly in the three treatment groups(P＞0.05). The challenge study with Aeromonas hydrophila showed that the three compounds, especially compound 3, could improve the livability of Amur sturgeon. The experiment results showed that compound 3 was a good Chinese herbal medicine immune enhancer which was efficient for Amur sturgeon to facilitate the non-specific immunity function.

Sun Yilei,Wang Di and Liu Hongbai. Effects of Compound Chinese Herbal Medicine on Non-specific Immunity of Amur Sturgeon[J]. Chinese Agricultural Science Bulletin, 2015, 31(8): 45-49.

References

[1] 田娟,冷向军,李小勤,等.复方中草药制剂对草鱼生长性能·肌肉成分和血清非特异性免疫的影响[J].安徽农业科学,2008,36(28):12282-12284.
[2] Wang T, Sun Y, Jin L, et al. Enhancement of non-specific immune response in sea cucumber (Apostichopus japonicus) by Astragalus membranaceus and its polysaccharides[J]. Fish & Shellfish Immunology, 2009,27(6):757-762.
[3] 刘红柏,黄江,马爱枝,等.中草药复方添加剂对施氏鲟生长及血液生化指标的影响[J].水产学杂志,2009,22(3):14-18.
[4] 王荻,刘红柏.中药复方A对施氏鲟生长及血液生化指标的影响[J].江苏农业科学,2013,41(9):205-207.
[5] Ni M, Wen H, Li J, et al. The physiological performance and immune responses of juvenile Amur sturgeon (Acipenser schrenckii) to stocking density and hypoxia stress[J]. Fish & Shellfish Immunology, 2014,36(2):325-335.
[6] 孙大江,曲秋芝,张颖,等.中国的鲟鱼养殖[J].水产学杂志,2011,24(4):67-70.
[7] 刘华忠,定忠,赵学明.复方中草药对彭泽鲫非特异性免疫功能的影响[J].淡水渔业,2004,34(3):31-32.
[8] 王俊相,李玉萍,孔令富,等.鱼类免疫系统的研究进展[J].四川畜牧兽医,2010,37(7):29-31.
[9] 李莉,李春梅.鱼类非特异性免疫研究进展[J].河南农业科学,2012,41(2):26-32.
[10] 李小梅,劳惠燕,王蔚淼,等.复合中草药对草鱼生长性能和非特异性免疫功能的影响[J].粮食与饲料工业,2013,12(12):49-51.
[11] 王荻,刘红柏.中草药方剂对施氏鲟非特异性免疫功能的影响[J].江西农业大学学报,2013,35(2):249-254.
[12] 宿斌,刘红柏.5种中草药对哲罗鲑生长率、死亡率以及部分生化指标的影响[J].中国农学通报,2013,29(26):31-35.
[13] Yin G, Jeney G, Racz T, et al. Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus[J]. Aquaculture, 2006,253(1-4):39-47.
[14] 宿斌,刘红柏.饲料中添加复方中草药对哲罗鱼生长、存活及部分生化指标的影响[J].水产学杂志,2013,26(2):35-39.
[15] 刘红柏,卢彤岩,张春燕,等.黄芪对史氏鲟抗氧化能力及免疫力的影响[J].大连水产学院学报,2006,21(3):231-235.
[16] Rauta P R, Nayak B, Das S. Immune system and immune responses in fish and their role in comparative immunity study: A model for higher organisms[J]. Immunology Letters, 2012,148(1):23-33.
[17] Boshra H, Li J, Sunyer J O. Recent advances on the complement system of teleost fish[J]. Fish & Shellfish Immunology, 2006, 20(2):239-262.
[18] 周显青,牛翠娟,孙儒泳.黄芪对中华鳖免疫和抗酸应激能力的影响[J].水生生物学报,2003,27(1):110-112.
[19] Zhao L, Sun J S, Sun L. The g-type lysozyme of Scophthalmus maximus has a broad substrate spectrum and is involved in the immune response against bacterial infection[J]. Fish & Shellfish Immunology, 2011,30(2):630-637.
[20] Magnadóttir B. Innate immunity of fish (overview)[J]. Fish & Shellfish Immunology, 2006, 20(2): 137-151.
[21] 艾春汉,邹金虎,喻运珍,等.6种中药对鲫非特异性免疫效果的影响[J].淡水渔业,2009,39(1):76-79.
[22] Zhang G, Gong S, Yu D, et al. Propolis and Herba Epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker, Myxocyprinus asiaticus[J]. Fish & Shellfish Immunology, 2009,26(3):467-472.
[23] Tang J, Cai J, Liu R, et al. Immunostimulatory effects of artificial feed supplemented with a Chinese herbal mixture on Oreochromis niloticus against Aeromonas hydrophila[J]. Fish & Shellfish Immunology, 2014,39(2):401-406.
[24] 张冰,林志健,翟华强,等.基于“三要素”假说研究中药药性的设想[J].中国中药杂志,2008,33(2):221-223.
[25] 王广军,谢骏,余德光,等.一氧化氮及一氧化氮合酶在水生动物免疫系统中的研究进展[J].水产科技,2009(3):1-6.
[26] Rubbo H. Nitric oxide and peroxynitrite in lipid peroxidation[J]. Medicina: B Aires, 1998,58(4):361-366.
[27] 李华,张太娥,李强.复方中草药对大菱鲆非特异性免疫力的影响[J].大连海洋大学学报,2013,28(2):115-120.
[28] Ardó L, Yin G, Xu P, et al. Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila[J]. Aquaculture, 2008,275(1-4):26-33.
[29] Yin G, Ardó L, Thompson K D, et al. Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophila[J]. Fish & Shellfish Immunology, 2008,26(1):140-145.