Abstract: The paper aims to study the influence of nitrogen and potassium rates on physiology characteristics of super rice ‘Guiliangyou 2’, in order to provide theory basis for super rice nitrogen and potassium comprehensive management. Field experiments were conducted to determine the effects of nitrogen and potassium rates on chlorophyll content, nitrate reductase activity, soluble protein content, glutamine synthetase activity and yield of super hybrid rice. Super hybrid rice cultivar ‘Guiliangyou 2’ was grown under three nitrogen rates (172.5, 207, 241.5 kg/hm²) and three potassium rates (216, 288, 360 kg/hm²). A treatment without nitrogen and potassium application (N0K0) was taken as a check. The results showed that, the experimental foundation soil yield was high, yield of N0K0 treatment was 10.01 t/hm². The results showed that applying nitrogen and potassium fertilizer increased chlorophyll content (6.44%-37.27%), nitrate reductase activity (19.33%-107.19%), soluble protein content (10.90%-36.43%), glutamine synthetase activity (14.25%-76.99%) and yield (9.39-10.63 t/hm²) of ‘Guiliangyou 2’ leaves significantly at different growth stages. However, no significant differences were found among the three nitrogen rates and among the three potassium rates. With high foundation soil yield like in the experiment, the sensitive degree of super rice physiology characteristics in applying nitrogen and potassium content is low. When nitrogen and potassium rates exceed 172.5 kg/hm² and 216 kg/hm², increasing nitrogen and potassium rates can not significantly improve physiological characteristics of super hybrid rice.