Abstract: In order to investigate the effects of different precipitation of Yunnan tobacco growing areas on field cultivation of tobacco, based on average rainfalls of 34 years (1971-2004) of Yunnan tobacco growing areas, four sites including Dongchuan District of Kunming City (the average rainfalls≤500 mm), Jiangchuan County (500-700 mm), Hongta District (500-700 mm) of Yuxi City and Pu’er County of Nin’er City (≥1100 mm) were chosen because their rainfall were gradually increased from north to south. According to their average rainfalls of 34 years, tobacco‘Yunyan 87’was planted with simulated rainfalls in tunnel house in Zhaowei flue-cured tobacco test base (24°18′N, 102°29′E, 1645 m a.s.l.) of Hongta area. With non-tunnel house planted tobaccos as control(CK), the effects of different simulated precipitation of 4 sites on the tobacco leaf δ¹³C values and phyto- physiological indicators (PPI) and their correlation were investigated. The results showed that the mean of five treatedδ¹³C value of tobacco leaves ranged from -26.99‰ to -25.34‰, and the values of leavesδ¹³C changed as follows: Jiangchuan(T2)＞Dongchuan(T1)＞Puer(T3)＞Zhaowei(T4)＞CK(T5). As for PPI, leaf mass per area-leaf (LMAL), chlorophyll content (Chl), malondialdehyde content (MDA), flavonoid content (FC) and soluble protein content (SPC) were selected to be determined. The LMAL of five treatments have no significant change, however the maximum Chl was found in CK, while that of T4, T1, T2 gradually decreased, and minimum Chl was found in T3; for MDA, T4 was the highest, and the order was T4＞T3＞T2＞ T1＞CK; for FC of tobacco, the order was T4＞T2＞T3＞T1＞CK; and for SPC of tobacco, the order was T4＞T3＞ T2＞CK＞T1. Further statistical analysis showed that tobacco leaf δ¹³C values had a remarkably positive correlation with SPC and total carbon respectively, and a positive correlation with the MDA and the entire nitrogen respectively with relatively weak relevance, while leafδ¹³C values had negative correlation with the FC and the ratio of carbon/ nitrogen respectively with very weak relevance. Among all treatment, T2 (Jiangchuan) displayed the strongest adaptability to climate with better growth and development under the moderate rainfall condition.