Effects of Chlorine Dioxide on Variation and Composition of Heterotrophic Bacteria Flora in Intestine of Grass Carp (Ctenopharyngodon idellus)

doi:10.11924/j.issn.1000-6850.casb14100113

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (8): 50-56.doi: 10.11924/j.issn.1000-6850.casb14100113

Special Issue: 水产渔业

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Effects of Chlorine Dioxide on Variation and Composition of Heterotrophic Bacteria Flora in Intestine of Grass Carp (Ctenopharyngodon idellus)

Zheng Zonglin^1,2, Wang Guangjun¹, Zheng Shuming², Zhu Chengke²

(¹Pearl River Fisheries Research Institute of Chinese Academy of Fishery Sciences, Guangzhou 510000;²Fisheries Breeding and Healthy Cultivation Research Centre, Southwest University, Chongqing 402460)

Received:2014-10-27 Revised:2015-03-03 Accepted:2015-01-09 Online:2015-04-07 Published:2015-04-07

Abstract

Abstract: To investigate the impacts of adding disinfectants in the water on the intestinal microflora composition of aquatic animals, two different methods (splashing chlorine dioxide and hanging chlorine dioxide in bags) were adopted in the grass carp pond to study the total number of heterotrophic bacteria and the composition of heterotrophic bacteria flora in pond water, sediment, and grass carp intestine. The trial lasted 30 days with 4 treatment groups. 250 g ClO₂ were splashed to the whole pool and hanged in bags respectively in group 1 and group 2. And 500 g ClO₂ were splashed to the whole pool and hanged in bags respectively in group 3 and group 4. The results showed that the total number of bacteria in pond water and sediment differed significantly with different ClO₂ using strategies. Among them, the total number of living bacteria in group 2 kept the highest in the three samples, whereas no significant differences were found among group 1, 3 and 4(P>0.05). Similar trend was found in the number variation of grass carp intestinal bacteria and aeromonas. Total number of intestinal bacteria and aeromonas in group 2 and group 4 ranked the first and the second, respectively. Total number of intestinal bacteria in group 2 was significantly higher than that in group 1, 3 and 4(P<0.05), and total number of aeromonas in group 2 and group 4 was also significantly higher than that in group 1 and 3(P<0.05). However, no significant difference was found between group 1 and group 3(P>0.05). Furthermore, 161 bacteria were isolated based on the physiological and biochemical identification. Aeromonas, Pseudomonas and Bacillus subtilis occupied 15.5%, 19.3% and 20.5% of the total intestinal living bacteria, respectively, and were found to be the dominant intestinal bacteria in grass carp. Besides, Shannon-wiener index in group 2 was significantly higher than that in other groups (P<0.05) and the index in group 4 and group 1 was significantly higher than that in group 3 (P<0.05), but no significant difference was found between group 4 and group 1(P>0.05). The results in this experiment effectively demonstrated that using chlorine dioxide in grass carp pond would have a significant impact on intestinal bacteria of grass carp.

Zheng Zonglin,Wang Guangjun,Zheng Shuming and Zhu Chengke. Effects of Chlorine Dioxide on Variation and Composition of Heterotrophic Bacteria Flora in Intestine of Grass Carp (Ctenopharyngodon idellus)[J]. Chinese Agricultural Science Bulletin, 2015, 31(8): 50-56.

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Effects of Chlorine Dioxide on Variation and Composition of Heterotrophic Bacteria Flora in Intestine of Grass Carp (Ctenopharyngodon idellus)

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