Abstract: The yield and stability of cotton cultivars, the pilot representative and discriminability, the ideal screening pilot and ideal cultivars, cultivars suitable for cultivation of a regional division were comprehensive evaluated by GGE biplot in Hunan Cotton Cultivar Regional Trail group B in 2013, in order to study the interaction relations of testing cultivars and the environment, scientifically evaluate testing varieties and testing sites, and provide the basis theory for cultivars assessment, cultivars application and suitable planting area selection. The results showed that: genotype, environment, genotype and environment interaction effect of lint yield reached a significant level, in which the main environmental effect, the main effect of genotype and genotype environment interaction effects accounted for 57.99% 13.54% and 28.48% of the sum of squares of variation respectively. We concluded that B3 was the most ideal variety for its high yielding, B5 was the best cultivar for its stable yielding, and Da Tonghu, Junshan and Hunan Cotton Science Institute were the most ideal test sites.