Abstract: To reduce the possibilities of genetic variation or vitrification during continuous subculture via regenerating protocorms and to alleviate the browning, a Phalaenopsis’ subculture technique via regenerating adventitious buds from vegetative buds produced from the flower stalks and applying anti-browning agent was studied. The results showed that: the highest vegetative bud induction rate was achieved when the middle auxiliary bud stem segments from the five-nodes flower stalks were inoculated in the medium of VW 6-BA 5 mg/L NAA 0.1 mg/L sugar 30 g/L. In subculture, TDZ was more effective than 6-BA in stimulating adventitious buds regeneration. The best subculture multiplication efficiency was achieved when the inoculating buds treated with ‘cutting leaf’ were cultured in the medium VW TDZ 1 mg/L citric acid 300 mg/L sugar 10 g/L coconut water 15%, or in the medium VW 6-BA 5 mg/L NAA 0.1 mg/L citric acid 300 mg/L sugar 10 g/L coconut water 15%. Through studies, the Phalaenopsis’ subculture multiplication system was preliminarily set up via regenerating adventitious buds.