Abstract: The study aims to clarify the effects of Cu and Zn pollution on growth of wheat, and provide theoretical guidance for the wheat production of high quality, efficiency and safety. By adding copper and zinc salts, the author carried out a pot experiment and studied chlorophyll fluorescence parameters and chlorophyll content, protective enzyme and yield in wheat leaves during filling stage of‘Jinmai96’ . Results showed that SPAD values of Cu and Zn treatments first increased and then decreased in blooming stage. Low concentrations of Cu and Zn treatments could increase chlorophyll content and delay senescence process. In late grouting stage, 100 mg/kg of Cu and Zn treatments had strong photochemistry activity and caused lesser injury; treatments higher than 100 mg/kg were not conducive to the maintenance of cell membrane stability in wheat leaves, suitable Cu and Zn could delay membrane peroxidation and cell aging process of wheat flag leaf lipid. CAT content of flag leaves of wheat first increased and then decreased from jointing to filling stage. 100 mg/kg of Cu and Zn had the highest CAT activity. Plant height was less affected by Cu and Zn. 100 mg/kg of Cu and Zn had the highest yield of 12.86 g and 12.17 g, respectively.