Abstract: The aim of the study was to investigate the effect of TLR2, TLR4 in injury of rat intestinal mucosa microvascular endothelial cells (RIMECs) induced by lipopolysaccharide (LPS). Rat intestinal mucosa microvascular endothelial cell in vitro was taken as a model, and different concentrations of LPS (1, 5, 10 μg/mL) were used to stimulate RIMECs for different time (3, 6, 9, 12, 24 h). Expressions of TLR2 and TLR4 on cell surface were detected by semi quantitative RT-PCR method. Then LPS at different concentrations (1, 5, 10 g/mL) were used to stimulate RIMECs for 9 h. Then expressions of TLR2 and TLR4 cells were detected by semi quantitative RT-PCR method. The results showed that expression of TLR2 and TLR4 increased when RIMECs were stimulated by LPS compared with the control group. Moreover, after stimulation with different concentrations of LPS for different time, expression of TLR2 mRNA and TLR4 mRNA basically reached the peak at 9 h, and the expression was time-independent. Expression of TLR2 mRNA was the highest when the concentration of LPS was 5 μg/mL after 9 h, and the expression was dose-independent. LPS at a concentration of 10 μg/mL stimulating RIMECs had the strongest effect in the expression of TLR4 mRNA, and was dose-dependent. The results demonstrated that TLR2 and TLR4 played an important role in the process of LPS damaging RIMECs.