Effect of Houttuynia cordata Floating-bed on the Structure and Function of Bacterial Community in the Sediments of Tilapia Aquacultural System

doi:10.11924/j.issn.1000-6850.casb14120173

Abstract

Abstract: To explore the effect of Houttuynia cordata floating-bed on bacterial community of sediment in tilapia pond, Biolog-Eco and PCR-DGGE were used to evaluate structure and function of the bacterial community in the sediments in Houttuynia-Tilapia symbiotic system from September to November in 2013. The Biolog-Eco results showed that microbial metabolic activity and bacterial diversity index in the treatment ponds were lower than those of the control ponds in September, while the status of the treatment and control ponds were just opposite from October to November. The richness indexes of the treatment ponds of different months were significantly different compared with that of the control ponds(P<0.05), therefore Houttuynia cordata floating-bed could effectively increase the bacterial biomass and species richness in the mid and late period. Principal component analysis (PCA) results showed that relative utilization ratios of carboxylic acids were significantly different in different months, and the different degrees appeared in the order of October>November>September. The DGGE results indicated that the composition of microbial community in the sediments of treatment and control ponds were similar. The bacteria belonged to six bacterial phyla, including Proteobacteria, Bacteroidetes, Acidobacteria, Chloroflexi, Cyanobacteria and Fusobacteria. Proteobacteria (54.17%) was the dominant bacterial community. However, the bacterial community diversity of the treatment ponds was obviously higher than that of the control ponds in the same month. As a result, Houttuynia cordata floating bed had a positive effect on optimizing the structure of microbial community, and increasing the diversity of microbial community.

Shi Lina,Ke Xiaoli,Liu Zhigang,Zhang Jiandong,Yao Zhenfeng,Ke Hao and Lu Maixin. Effect of Houttuynia cordata Floating-bed on the Structure and Function of Bacterial Community in the Sediments of Tilapia Aquacultural System[J]. Chinese Agricultural Science Bulletin, 2015, 31(14): 64-73.

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