Abstract: A comparison between two emasculation methods in timing, percentage of style injury and fruit set in 20 days after pollination was conducted to find out the simple and practical way for artificial emasculation in Macadamia, with cultivar ‘Renown’ as the mother tree. The flowers at different anthesis were chosen to test the two methods. The result showed that emasculation methods with perianth were better than needle slitting. It only took 10 minutes in emasculating with perianth when the perianth tube splits in the first day, with 11.1% of style tip injury and 74.1% of set in 20 days of pollination. While the timing, injury rate and the set were 36.0 min, 36.3% and 27.8% respectively in needle slitting. In emasculation method with perianth, the optimum conditions were to choose those perianth tube splits in the first day and carry out the emasculation after ring cut the base of tube in half an hour, and pollinated in 48 hours. This method is simple and easy, which can produce no false hybrid, enhance the purity of hybrid, and significantly improve crossbreeding efficiency in Macadamia.