Spatial-temporal Expression of CC-NBS-LRR Protein-coding Gene Resisting to Infection of Aphelenchoides besseyi in Oryza sativa L. ssp. Indica

doi:10.11924/j.issn.1000-6850.casb15010080

Abstract

Abstract: Os11gRGA8 gene was cloned and its bioinformatics were analyzed in order to explore the molecular mechanism of the indica rice (Oryza sativa L. ssp. Indica) and the role of resistance genes in the course of illness. Bioinformatics of Os11gRGA8 gene was analyzed, and its function in the process of Aphelenchoides besseyi infection was studied. Os11gRGA8 genes were in chromosome 11, containing 733 amino acids, its coding protein isoelectric point was 5.99 and the relative molecular mass was 84355.07. The sequence analysis showed that four structure domains RX- CC, AAA_22, NBS and LRR_4 were contained in its amino acid sequence and the disease-resistant protein encoded by Os11gRGA8 gene belonged to CC-NBS-LRR diseaseresistant protein family. Q- PCR results showed that, compared with CK rice seedlings group, the Indica inoculated by A. besseyi SAMN02420038 got a raised quantity of Os11gRGA8 gene expression and within 24 h the increase was relatively slow. Then the growth rose suddenly in 48 h, but the increase declined in 72 h,indicating that Os11gRGA8 gene was involved in Indica natural immune reaction process and played a role in the process of A. besseyi resistance infection.

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