Abstract: An experiment of fertilization effect on rice was conducted according to the theory of“3414” regression optimum design to explore the index system of fertilization in Daan and to guide fertilization on rice. The results showed that, compared with CK, yield of each fertilization treatment was enhanced. The N2P2K1 treatment could obtain the highest yield of 10336 kg/hm² and the best economic benefit of 16885.07 yuan/hm² among the 14 treatments. The N, P, K quadratic was established as the objective function of rice yield. Establishment of fertilization index by regression model of N, P, K was N 203.99 kg/hm², P 102.08 kg/hm² and K 83.29 kg/hm², and the highest yield of rice was 10471.23 kg/hm². Combined with the local fertilizer prices, the optimum fertilizing amount was N 159.11 kg/hm², P 82.35 kg/hm² and K 54.41 kg/hm², under which the yield could reach 10323.38 kg/hm², and this fertilizing formula could be recommended in local rice production.