Genetic Variation Analysis of Ziziphus jujuba ‘Zanhuang Dazao’ by SSR Markers

doi:10.11924/j.issn.1000-6850.casb15010121

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (16): 94-100.doi: 10.11924/j.issn.1000-6850.casb15010121

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Genetic Variation Analysis of Ziziphus jujuba ‘Zanhuang Dazao’ by SSR Markers

Gao Jiao¹, Liu Jun¹, Shen Lianying², Chu Xinfang³, Xu Chaoqun¹, Du Zengfeng⁴, Li Dengke⁵, Wang Zhe¹, Zhang Zhendong¹, Pang Xiaoming¹

(¹National Engineering Laboratory for Tree Breeding/ College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083; ²Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding Hebei 071001;³Zan huang Forestry Bureau, Shijiazhuang 051230; ⁴National Improved Cultivar Station of Jujube, Cangzhou Heibei 061000; ⁵Pomology Institute, Shanxi Academy of Agricultural Science, Taigu Shanxi 030815)

Received:2015-01-18 Revised:2015-05-22 Accepted:2015-03-23 Online:2015-07-27 Published:2015-07-27

Abstract

Abstract: Information about the genetic variation of Ziziphus jujuba ‘Zanhuang Dazao’ at molecular level can provide effective basis for selective breeding of new cultivars. 395 samples of Ziziphus jujuba ‘Zanhuang Dazao’ were collected and were amplified by PCR using 16 SSR markers. The genetic relationships among them were investigated based on analysis of fingerprint data after capillary electrophoresis. Totally, 51 alleles were detected and the number of alleles amplified by per primer ranged from 2 to 9 with an average value of 3.2. The sizes of PCR products ranged from 104 bp to 304 bp. The amount of rare alleles was 16 and polymorphic loci percentage was 75%. After clustering, 28 unique genotypes which were different from each other were obtained within 395 samples, which meant 28 different genotypes were distinguished. Further cluster analysis showed that genetic similarities among these 28 genotypes ranged from 0.7500 to 0.9804 with an average of 0.9047. At the genetic similarity of 0.90, the genotypes were classified into 5 groups. The results indicated that there was a certain degree of genetic variation among Ziziphus jujuba ‘Zanhuang Dazao’ and still had potential to select new cultivars with better quality.

Gao Jiao,Liu Jun,Shen Lianying,Chu Xinfang,Xu Chaoqun,Du Zengfeng,Li Dengke,Wang Zhe,Zhang Zhendong and Pang Xiaoming. Genetic Variation Analysis of Ziziphus jujuba ‘Zanhuang Dazao’ by SSR Markers[J]. Chinese Agricultural Science Bulletin, 2015, 31(16): 94-100.

References

[1] 崔丽贤,刘金利,王学军.河北赞皇大枣发展问题与对策[J].现代园艺,2014,9:17-18.
[2] 刘学生,陈龙,王金鑫,等.‘苹果枣’自然三倍体倍性的发现与鉴定[J].园艺学报,2013,40(3):426-432.
[3] 彭建营,刘平,周俊义,等.‘赞皇大枣’不同株系的染色体数及其核型分析[J].园艺学报,2005,32(5):798-801.
[4] 曲泽洲,王永蕙,温陟良.三倍体赞皇大枣的核型研究[J].河北果树,1990(4):23-24.
[5] 许晓光,陈龙,彭建营.酸枣和赞皇大枣花粉母细胞减数分裂的观察[J].园艺学报,2009,36(8):1127-1133.
[6] 刘平,彭建营,彭士琪,等.赞皇大枣的群体遗传多样性评价[J].果树学报,2006,23(5):685-689.
[7] 赵锦,刘孟军.赞皇大枣优良品系及赞新大枣的RAPD分析[J].林业科学,2003,39(3):153-156.
[8] 刘平.赞皇大枣及相关类群群体遗传变异的分子评价[D].保定:河北农业大学,2002.
[9] 王永蕙,温陟良.三倍体赞皇大枣的形态特征[J].落叶果树,1990,22(4):1-3.
[10] 王艳,彭建营.‘赞皇大枣’增殖培养研究[J].中国农学通报,2011,27(16):210-214.
[11] 宋锋惠,张萍,罗青红,等.新疆不同种植区赞皇大枣品质的差异分析[J].经济林研究,2010,28(3):105-108.
[12] 杨海旭,王洋,赵彦檩,等.赞皇大枣枣疯病植原体分子分类[J].中国农业科学,2011,44(21):4429-4437.
[13] Ellegren Hans. Microsatellites: simple sequences with complex evolution[J]. Nature reviews Genetics,2004,5(6):435-445.
[14] 侯静,马秋月,陈赢男,等.枣树品种鉴别适宜方法的讨论.[J].种子,2014,33(2):74-77.
[15] 殷晓,张春梅,李新岗,等.陕北枣品种群遗传结构的SSR分析[J].西北农林科技大学学报:自然科学版,2014,42(6):152-160,167.
[16] 张春梅,殷晓,李新岗,等.黄河沿岸酸枣遗传多样性研究[J].西北农林科技大学学报:自然科学版,2013,41(12):107-112.
[17] 殷晓.基于SSR标记的中国枣遗传多样性研究[D].陕西杨凌:西北农林科技大学,2013.
[18] 张春梅.酸枣SSR引物开发及遗传多样性研究[D].陕西杨凌:西北农林科技大学,2013.
[19] 麻丽颖,孔德仓,刘华波,等.36份枣品种SSR指纹图谱的构建[J].园艺学报,2012,39(4):647-654．
[20] 麻丽颖.部分枣种质资源SSR指纹图谱的构建[D].北京:北京林业大学,2012.
[21] 王斯琪,唐诗哲,孔德仓,等.利用SSR标记进行枣树子代苗父本鉴定[J].园艺学报,2012,39(11):2133-2141．
[22] 王斯琪.‘冬枣’×‘映山红’遗传连锁图谱构建[D].北京:北京林业大学,2013.
[23] 许莉斯.枣果实性状QTL定位及优良基因型筛选研究[D].河北保定:河北农业大学,2012.
[24] 杨影.我国枣树遗传改良的研究进展[J].吉林林业科技,2014,43(5):43-46.
[25] Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of leaf tissue[J]. Phytochem Bull,1987,19:11-15.
[26] Wang S Q, Liu Y, Ma L Y, et al. Isolation and characterization of microsatellite markers and analysis of genetic diversity in Chinese Jujube (Ziziphus jujuba Mill.)[J]. PLoS ONE,2014,9(6):e99842.
[27] Schuelke M. An economic method for the fluorescent labeling of PCR fragments[J]. Nature biotechnology,2000,18(2):233-234.
[28] Amos W, Hoffman J, Frodsham A, et al. Automated binning of microsatellite alleles: problems and solutions[J].Molecular Ecology Notes,2007,7:10-14.
[29] 庞晓明,邓秀新,胡春根.枳属36份特异种质的AFLP指纹图谱构建与分析[J].园艺学报,2003,30(4):394-398.
[30] 李登科,王永康,隋串玲,等.大果型制干加工枣新品种——晋赞大枣的选育[J].果树学报,2011,28(6):1130-1131.
[31] 曲泽洲,王永惠.中国果树志·枣卷[M].北京:中国林业出版社,1993:191.

Genetic Variation Analysis of Ziziphus jujuba ‘Zanhuang Dazao’ by SSR Markers

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