Abstract: The objective of this study was to separate Inv gene from ougan fruit and get sequence information, which would be used in further study on sucrose metabolism of ougan fruit at molecular level. Methods of homologous cloning and RT-PCR were used for Inv gene clone from ougan fruit. The gene we got was named CrInv1 and the accession number was KF694988. Bioinformatics methods were used to get information of predicted Inv protein including relative molecular mass, isoelectric point (pI), the conserved domains and phytogenetic analysis results. Results showed that CrInv1 was 1932 bp in size, encoding a putative protein of 643-amino acid. Sequence alignment results showed that CrInv1 was highly conserved among different plants, and shared more than 70% sequence homology with other plants from NCBI. With the calculation by software Primer 5.0, relative molecular mass of the putative CrInv1 protein turned out to be 72.18 kDa, and the pI was 6.882. Results indicated that CrInv1 from ougan fruit belonged to neutral invertase and it was rich in nonpolar amino acids. The subcellular localization of predicted CrInv1 protein was within the cell membrane. Phylogenetic analysis showed that putative CrInv1 protein was not grouped with that from grape, apple and peach, and was more close to that from lichi and strawberry. The study got Inv gene from ougan fruit and the sequence information was analyzed. The results lay a foundation for subsequent research on sucrose metabolism of ougan fruit.